To study the interrelations between the rs4073 polymorphism of the IL-8 gene, IL-8 serum levels and severity of alcoholic hepatitis (AH).
Material and methods
We enrolled 93 patients with AH (age 49.5±9.1 years, 77% males). The severity of AH was evaluated using the Maddray score discriminant function (MDF). Sixty six patients had mild AH (MDF 8.0±1.1), whereas 27 patients presented with severe AH (MDF 63.0±15.3). Twenty four alcohol abusers without liver disease formed the control group. Serum IL-8 levels were measured with ELISA. Real time PCR was used to determine the allelic variants of the rs4073 IL-8 gene.
Serum IL-8 levels in patients with severe AH were significantly higher than in patients with mild AH and control patients. Severe AH was associated with the threshold IL-8 level >71.8 pg/ml with 62.5% sensitivity and 77.8% specificity. Multivariate correlation analysis showed no association between IL-8 levels and any clinical, laboratory or instrumental parameters in the studied groups. IL-8 rs4073 genotype ТТ and allele Т were more frequent in patients with mild and severe AH compared to genotype АА and allele А, respectively. Moreover, heavy drinkers carrying genotype ТТ and allele Т had significantly higher risk of AH compared to genotype AA and allele A carriers. Significant association was found between high serum IL-8 levels and carriage of the T allele and TT genotype among patients with severe AH.
Serum IL-8 concentration was increased in patients with severe AH and did not correlate with routine clinical or laboratory parameters. High IL-8 levels were associated with carriage of allele T and genotype TT of the rs4073 IL-8 gene in patients with severe AH. Genotype TT and T allele were more prevalent in AH and increased the risk of its development.
Alcoholic hepatitis, IL-8, rs473, IL-8 gene.